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Clean blood spills properties Limited use as disinfectant
because of narrow
microbiocidal spectrum
Source: Handwashing, Cleaning, Disinfection and Sterilization in Health Care. CCDR 24S8,
December 1998: Health Canada.
13
Selection and Use of Disinfectants
Appendix 3.
Directions for Preparing and Using Chlorine-based Disinfectants
Product Intended use Dilution Available chlorine
Household bleach Cleanup blood spills1 1 part bleach to 9 0.5%
(5% sodium parts water 5000 ppm
hypochlorite with Surface Disinfection2 1 part bleach to 50 0.1%
50000 ppm) parts water approx. 1000 ppm
Food Surfaces3 1 part bleach to 200 0.025%
parts water approx. 200 ppm
Instruments/surfaces 1 part bleach to 1 part 2.5 to 5%
contaminated with water / undiluted 20000 to 50000 ppm
tissue infective for
CJD4
Sodium Cleanup blood spills Dissolve 8.5 g in one 0.85% or 5000 ppm
dichloroisocyanurate litre of water
(NaDCC) powder
with 60% available
chlorine
Chloramine-T powder Cleanup blood spills Dissolve 20 g in one 2% or 5000 ppm
with 25% available litre of water
chlorine
1. Contact time at least 10 minutes.
2. Contact time at least 5 minutes. Wet surface with bleach solution and allow drying.
3. Contact time at least 2 minutes. During gastroenteritis outbreaks 1:50 dilution is
recommended.
4. Contact time 1 hour, then rinse. Instruments require sterilization following disinfection.
14
Selection and Use of Disinfectants
7. References
1. DC Drummond. The Prevention of Cross Infection in the Physician s Office. College of
Physicians and Surgeons of BC, February 1992.
2. Guidelines for Infection Control Practice. APIC Guideline for Selection and Use of
Disinfectants. AJIC 1996; 24: 313-342
3. Infection Control Guidelines. Handwashing, Cleaning, Disinfection and Sterilization in
Health Care. CCDR 24S8, December 1998: Health Canada.
4. Infection Control Guidelines. Classic Creutzfeldt-Jakob disease in Canada. CCDR 2002;
28S5: 1-84. Health Canada.
5. J Kennedy, J Bek. Selection and Use of Disinfectants. Nebraska Cooperative Extension 1998
6. WA Ratula, DJ Weber. Infection Control: the role of disinfection and sterilization. ICHE
December 1999 20(12): 821-7.
7. WA Ratula, DJ Weber. Uses of inorganic hypochlorite (bleach) in health care facilities. CMR
1997; 10: 597-610.
8. WA Rutala, DJ Weber. Draft Guideline for Disinfection and Sterilization in Healthcare
Facilities. CDC Healthcare Infection Control Practices Advisory Committee.2001
9. SA Sattar et al. Products based on accelerated and stabilized hydrogen peroxide. Evidence
for broad-spectrum germicidal activity. Virox Technologies, 2002.
10. SA Sattar. Evaluation of effectiveness of a 0.5% formulation of accelerated hydrogen
peroxide against human rhinovirus, feline calicivirus and human rotavirus. Virox
Technologies, October 2002.
15
Selection and Use of Disinfectants
Prepared by:
Bruce Gamage
Infection Control Consultant
Laboratory Services, BCCDC
Reviewed by:
Dr. Martin Petric
Clinical Virologist
Dr. Gwen Stephens
Medical Microbiologist
Lorraine McIntyre
GI Outbreak Coordinator
Laboratory Services, BCCDC
Joe Fung
Supervisor, Environmental Services
BC Professionals in Infection Control
Approved by:
Dr. Judy Isaac-Renton
Director, Laboratory Services
BCCDC
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